With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation using murine splenocyte cells. The test formulation was divided into two parts. One was the control without the Biofield Energy Treatment. The other part was labelled the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers. Through MTT assay, all the test formulation concentrations from 0.00001053 to 10.53 µg/mL were found to be safe with cell viability ranging from 102.61% to 194.57% using splenocyte cells. The Biofield Treated test formulation showed a significant (p≤0.01) inhibition of TNF-α expression by 15.87%, 20.64%, 18.65%, and 20.34% at 0.00001053, 0.0001053, 0.01053, and 0.1053, µg/mL, respectively as compared to the vehicle control (VC) group. The level of TNF-α was reduced by 8.73%, 19.54%, and 14.19% at 0.001053, 0.01053, and 0.1053 µg/mL, respectively in the Biofield Treated test formulation compared to the untreated test formulation. The expression of IL-1β reduced by 22.08%, 23.69%, 23.00%, 16.33%, 25.76%, 16.10%, and 23.69% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, 1.053 and 10.53 µg/mL, respectively compared to the VC. Additionally, the expression of MIP-1α significantly (p≤0.001) reduced by 13.35%, 22.96%, 25.11%, 22.71%, and 21.83% at 0.00001053, 0.0001053, 0.01053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation significantly down-regulated the MIP-1α expression by 10.75%, 9.53%, 9.57%, and 10.87% at 0.00001053, 0.01053, 0.1053 and 1.053 µg/mL, respectively compared to the untreated test formulation. The results showed the IFN-γ expression was also significantly (p≤0.001) reduced by 39.16%, 40.34%, 27.57%, 26.06%, 42.53%, and 48.91% at 0.0001053, 0.001053, 0.01053, 0.1053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation showed better suppression of IFN-γ expression by 15.46%, 13.78%, 17.14%, and 13.11% at concentrations 0.001053, 0.01053, 0.1053, and 10.53 µg/mL, respectively compared to the untreated test formulation. Overall, the results demonstrated that The Trivedi Effect®- Biofield Energy Healing (TEBEH) has the capacity to potentiate the immunomodulatory and anti-inflammatory activity of the test formulation. Biofield Energy may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.
Published in | International Journal of Biomedical Engineering and Clinical Science (Volume 2, Issue 1) |
DOI | 10.11648/j.ijbecs.20160201.12 |
Page(s) | 8-17 |
Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Biofield Energy Healing Treatment, Biofield Energy Healers, The Trivedi Effect®, Herbomineral Formulation, Immune-Modulation, Pro-inflammatory Cytokines, Splenocytes
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APA Style
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, et al. (2017). Modulation of Pro-inflammatory Cytokines Expression of the Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Mouse Splenocytes. International Journal of Biomedical Engineering and Clinical Science, 2(1), 8-17. https://doi.org/10.11648/j.ijbecs.20160201.12
ACS Style
Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; William Dean Plikerd, et al. Modulation of Pro-inflammatory Cytokines Expression of the Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Mouse Splenocytes. Int. J. Biomed. Eng. Clin. Sci. 2017, 2(1), 8-17. doi: 10.11648/j.ijbecs.20160201.12
AMA Style
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, et al. Modulation of Pro-inflammatory Cytokines Expression of the Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Mouse Splenocytes. Int J Biomed Eng Clin Sci. 2017;2(1):8-17. doi: 10.11648/j.ijbecs.20160201.12
@article{10.11648/j.ijbecs.20160201.12, author = {Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and William Dean Plikerd and Peter L. Surguy and Robert John Kock and Rolando Baptista Piedad and Russell Phillip Callas and Sakina A. Ansari and Sandra Lee Barrett and Sara Friedman and Steven Lee Christie and Su-Mei Chen Liu and Susan Elizabeth Starling and Susan Jones and Susan Mardis Allen and Susanne Kathrin Wasmus and Terry Ann Benczik and Thomas Charles Slade and Thomas Orban and Victoria L. Vannes and Victoria Margot Schlosser and Yusif Sarkis Yamin Albino and Sambhu Charan Mondal and Snehasis Jana}, title = {Modulation of Pro-inflammatory Cytokines Expression of the Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Mouse Splenocytes}, journal = {International Journal of Biomedical Engineering and Clinical Science}, volume = {2}, number = {1}, pages = {8-17}, doi = {10.11648/j.ijbecs.20160201.12}, url = {https://doi.org/10.11648/j.ijbecs.20160201.12}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbecs.20160201.12}, abstract = {With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation using murine splenocyte cells. The test formulation was divided into two parts. One was the control without the Biofield Energy Treatment. The other part was labelled the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers. Through MTT assay, all the test formulation concentrations from 0.00001053 to 10.53 µg/mL were found to be safe with cell viability ranging from 102.61% to 194.57% using splenocyte cells. The Biofield Treated test formulation showed a significant (p≤0.01) inhibition of TNF-α expression by 15.87%, 20.64%, 18.65%, and 20.34% at 0.00001053, 0.0001053, 0.01053, and 0.1053, µg/mL, respectively as compared to the vehicle control (VC) group. The level of TNF-α was reduced by 8.73%, 19.54%, and 14.19% at 0.001053, 0.01053, and 0.1053 µg/mL, respectively in the Biofield Treated test formulation compared to the untreated test formulation. The expression of IL-1β reduced by 22.08%, 23.69%, 23.00%, 16.33%, 25.76%, 16.10%, and 23.69% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, 1.053 and 10.53 µg/mL, respectively compared to the VC. Additionally, the expression of MIP-1α significantly (p≤0.001) reduced by 13.35%, 22.96%, 25.11%, 22.71%, and 21.83% at 0.00001053, 0.0001053, 0.01053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation significantly down-regulated the MIP-1α expression by 10.75%, 9.53%, 9.57%, and 10.87% at 0.00001053, 0.01053, 0.1053 and 1.053 µg/mL, respectively compared to the untreated test formulation. The results showed the IFN-γ expression was also significantly (p≤0.001) reduced by 39.16%, 40.34%, 27.57%, 26.06%, 42.53%, and 48.91% at 0.0001053, 0.001053, 0.01053, 0.1053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation showed better suppression of IFN-γ expression by 15.46%, 13.78%, 17.14%, and 13.11% at concentrations 0.001053, 0.01053, 0.1053, and 10.53 µg/mL, respectively compared to the untreated test formulation. Overall, the results demonstrated that The Trivedi Effect®- Biofield Energy Healing (TEBEH) has the capacity to potentiate the immunomodulatory and anti-inflammatory activity of the test formulation. Biofield Energy may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.}, year = {2017} }
TY - JOUR T1 - Modulation of Pro-inflammatory Cytokines Expression of the Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Mouse Splenocytes AU - Mahendra Kumar Trivedi AU - Alice Branton AU - Dahryn Trivedi AU - Gopal Nayak AU - William Dean Plikerd AU - Peter L. Surguy AU - Robert John Kock AU - Rolando Baptista Piedad AU - Russell Phillip Callas AU - Sakina A. Ansari AU - Sandra Lee Barrett AU - Sara Friedman AU - Steven Lee Christie AU - Su-Mei Chen Liu AU - Susan Elizabeth Starling AU - Susan Jones AU - Susan Mardis Allen AU - Susanne Kathrin Wasmus AU - Terry Ann Benczik AU - Thomas Charles Slade AU - Thomas Orban AU - Victoria L. Vannes AU - Victoria Margot Schlosser AU - Yusif Sarkis Yamin Albino AU - Sambhu Charan Mondal AU - Snehasis Jana Y1 - 2017/01/07 PY - 2017 N1 - https://doi.org/10.11648/j.ijbecs.20160201.12 DO - 10.11648/j.ijbecs.20160201.12 T2 - International Journal of Biomedical Engineering and Clinical Science JF - International Journal of Biomedical Engineering and Clinical Science JO - International Journal of Biomedical Engineering and Clinical Science SP - 8 EP - 17 PB - Science Publishing Group SN - 2472-1301 UR - https://doi.org/10.11648/j.ijbecs.20160201.12 AB - With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation using murine splenocyte cells. The test formulation was divided into two parts. One was the control without the Biofield Energy Treatment. The other part was labelled the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers. Through MTT assay, all the test formulation concentrations from 0.00001053 to 10.53 µg/mL were found to be safe with cell viability ranging from 102.61% to 194.57% using splenocyte cells. The Biofield Treated test formulation showed a significant (p≤0.01) inhibition of TNF-α expression by 15.87%, 20.64%, 18.65%, and 20.34% at 0.00001053, 0.0001053, 0.01053, and 0.1053, µg/mL, respectively as compared to the vehicle control (VC) group. The level of TNF-α was reduced by 8.73%, 19.54%, and 14.19% at 0.001053, 0.01053, and 0.1053 µg/mL, respectively in the Biofield Treated test formulation compared to the untreated test formulation. The expression of IL-1β reduced by 22.08%, 23.69%, 23.00%, 16.33%, 25.76%, 16.10%, and 23.69% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, 1.053 and 10.53 µg/mL, respectively compared to the VC. Additionally, the expression of MIP-1α significantly (p≤0.001) reduced by 13.35%, 22.96%, 25.11%, 22.71%, and 21.83% at 0.00001053, 0.0001053, 0.01053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation significantly down-regulated the MIP-1α expression by 10.75%, 9.53%, 9.57%, and 10.87% at 0.00001053, 0.01053, 0.1053 and 1.053 µg/mL, respectively compared to the untreated test formulation. The results showed the IFN-γ expression was also significantly (p≤0.001) reduced by 39.16%, 40.34%, 27.57%, 26.06%, 42.53%, and 48.91% at 0.0001053, 0.001053, 0.01053, 0.1053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation showed better suppression of IFN-γ expression by 15.46%, 13.78%, 17.14%, and 13.11% at concentrations 0.001053, 0.01053, 0.1053, and 10.53 µg/mL, respectively compared to the untreated test formulation. Overall, the results demonstrated that The Trivedi Effect®- Biofield Energy Healing (TEBEH) has the capacity to potentiate the immunomodulatory and anti-inflammatory activity of the test formulation. Biofield Energy may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life. VL - 2 IS - 1 ER -